Rabbit Anti-NALP6 (NACHT, LRR and PYD Domains-containing Protein 6, PYRIN-containing APAF1-like Protein 5, NLRP6, PYPAF5)
NALP proteins include the apoptosis regulator APAF1 (apoptotic protease activating factor 1) and mammalian NOD-LRR proteins and are thought to be involved in inflammation and reproduction. NALP6, also known as PYPAF5, is highly expressed in granulocytes and T cells and colocalizes with the PYRIN-CARD protein ASC. Co-expression of NALP6 with ASC results in a synergistic activation of NF-kB and activated caspase-1-dependent cytokine processing, suggesting that NALP6 functions in immune cells to coordinate the transduction of pro-inflammatory signals.
Applications
Suitable for use in Immunofluorescence, ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
Human Heart Tissue Lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to 16aa from near the N-terminus of human NALP6.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human NALP6.
