Technical Data

146746
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E IC IF WB
Crossreactivity
Hu Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-PAPPA2 (Pappalysin-2, Pregnancy-associated Plasma Protein A2, PAPP-A2, Pregnancy-associated Plasma Protein E1, PAPP-E, PLAC3)

PLAC3 (placenta-specific protein 3), also known as PAPP-A2, is a metalloproteinase that cleaves the insulin-like growth factor-binding protein (IGFBP)-5 in a IGF-independent fashion. It has been suggested that the cleavage of IGFBP-5 and to a lesser extent IGFBP-3 produced in the maternal decidua promotes feto-placental growth. Recent studies have shown elevated levels of PLAC3 in hypertensive disorders of pregnancy including preeclampsia and HELLP (hemolysis, elevated liver enzymes, and low platelets) syndrome.

Applications
Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunocytochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1ug/ml Immunocytochemistry: 10ug/ml Immunofluorescence: 20ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
HeLa Cell Lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to 15aa from near the N-terminus of human PLAC3. Epitope: aa450 -500
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human PLAC3. Species Crossreactivity: mouse and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Overgaard MT, Boldt HB, Laursen LS, et al. Pregnancy-associated plasma protein-A2 (PAPP-A2), a novel insulin-like growth factor-binding protein-5 proteinase. J. Biol. Chem.2001; 276:1849-53. 2. Wang J, Qiu Q, Haider M, et al. Expression of pregnancy-associated plasma protein A2 during pregnancy in human and mouse. J. Endocrin.2009; 202:337-45. 3. Nishizawa H, Pryor-Koishi K, Suzuki M, et al. Increased levels of pregnancy-associated plasma protein-A2 in the serum of pre-eclamptic patients. Mol. Hum. Reprod.2008; 14:595-602. 4. Buimer M, Keijser R, Jebbink JM, et al. Seven placental transcripts characterize HELLP syndrome. Placenta2008; 29:444-53.
USBio References
No references available
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