Rabbit Anti-EFCAB4B (EF-hand Calcium-binding Domain-containing Protein 4B, Calcium Release-activated Calcium Channel Regulator 2A, CRAC Channel Regulator 2A, CRACR2A, DKFZp686G13246, FLJ33046, FLJ33805, MGC4266)
EFCAB4B, also known as Calcium release-activated calcium channel regulator 2A, is a novel Ca2+-binding EF-hand protein that is thought to play a key role in store-operated Ca2+ entry in T-cells by regulating CRAC channel activation. EFCAB4B acts as a cytoplasmic calcium-sensor that forms a complex with ORAI1 and STIM1 at the junctional regions between the plasma membrane and the endoplasmic reticulum upon low Ca2+ concentration. A closely related protein, EFCAB4A, is likely to play a similar role as EFCAB4B, but the detailed function of EFCAB4A is still under investigation.
Applications
Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1ug/ml Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
Mouse Kidney Tissue Lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to 14aa from near the C-terminus of human EFCAB4B.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human EFCAB4B. Species Crossreactivity: mouse.
