Technical Data

146792
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E IF WB
Crossreactivity
Hu Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-ZMYM3 (Zinc Finger MYM-type Protein 3, Zinc Finger Protein 261, DXS6673E, KIAA0385, ZNF261, ZNF198L2)

Zinc-finger proteins contain DNA-binding domains characterized by the unique role of zinc and have a wide variety of functions such as transcriptional activation or repression. The protein folding and the DNA binding ability are governed by the coordination of a zinc ion. As a member of the MYM (myeloproliferative and mental retardation) gene family, ZMYM3 is highly conserved in vertebrates and most abundantly expressed in the brain. The encoded protein is a component of histone deacetylase-containing multiprotein complexes that function through modifying chromatin structure to keep genes silent. A chromosomal translocation (X;13) involving this gene is associated with X-linked mental retardation.

Applications
Suitable for use in Immunofluorescence, ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
Human Brain Tissue Lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to 20aa from near the C-terminus of human AXIN2.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human AXIN2. Species Crossreactivity: mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Rosenfeld R and Margalit H. Zinc fingers: conserved properties that can distinguish between spurious and actual DNA-binding motifs. J. Biomol. Struct. Dyn.1993; 11:557-70. 2. Gregory SG, Barlow KF, McLay KE, et al. The DNA sequence and biological annotation of human chromosome 1. Nature2006; 441:315-21 3. Sohal J, Reiter A, Goldman JM, et al. Cloning of ZNF237, a novel member of the MYM gene family that maps to human chromosome 13q11q12. Cytogenet. Cell Genet.2000; 89:24-8. 4. van der Maarel SM, Scholten IH, Huber I, et al. Cloning and characterization of DSX6673E, a candidate gene for X-linked mental retardation in Xq13.1. Hum. Mol. Gen.1996; 5:887-97.
USBio References
No references available
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