Rabbit Anti-RABGEF1 (Rab5 GDP/GTP Exchange Factor, Rabaptin-5-associated Exchange Factor for Rab5, RABEX5, Rabex-5, RAP1)
RABEX5 is a guanine nucleotide exchange factor (GEF) that forms a complex with rabaptin-5 and specifically activates Rab5, a small GTPase that plays an essential role in endocytic traffic. RABEX-5 is thought to also interact with another member of the Rab family, Rab22, which targets RABEX5 to early endosomes, allowing it to activate Rab5 and thereby promote Rab5-mediated endosome fusion.
Applications
Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1ug/ml Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
Rat Brain Tissue Lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to 19aa from near the internal region of human RABEX5.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human RABEX5. Species Crossreactivity: mouse and rat.
