Technical Data

146877
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
13L6
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-ENDOG (Endonuclease G, Mitochondrial, Endo G)

The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (EndoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD. EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans. EndoG also cleaves DNA in vitro.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 5-10ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
HepG2 Cell Lysate or EndoG Recombinant Protein
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to aa51-140 from human EndoG.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human EndoG. Species Crossreactivity: mouse and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Li LY, Luo X, Wang X. Endonuclease G is an apoptotic DNase when released from mitochondria. Nature 2001; 412:95-9. 2. Parrish J, Li L, Klotz K, et al. Mitochondrial endonuclease G is important for apoptosis in C. elegans. Nature 2001; 412:90-4 3. Hengartner MO. Apoptosis. DNA destroyers. Nature 2001; 412:27, 29. 4. Widlak P, Li LY, Wang X, et al. Action of recombinant human apoptotic endonuclease G on naked DNA and chromatin substrates: cooperation with exonuclease and DNase I. J. Biol. Chem. 2001; 276:48404-9.
USBio References
No references available
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