Goat Anti-HAMP (Hepcidin, Liver-expressed Antimicrobial Peptide 1, LEAP-1, Putative Liver Tumor Regressor, PLTR, HEPC, LEAP1, UNQ487/PRO1003, Hepcidin-25, Hepc25, Hepcidin-20, Hepc20)
Hepcidin, an human antimicrobial peptide, contains a leader sequence, a proteolysis site and a C-terminal 25aa active peptide. This peptide is most active against gram-positive bacteria, but also inhibits the growth of certain yeast and gram-negative bacteria. HAMP also functions as a signaling molecule involved in the maintenance of iron homeostasis. In conjugation with HFE it seems to regulate both intestinal iron absorption and iron storage in macrophages. Mutations in HAMP are found in juvenile hemochromatosis.
Applications
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Peptide ELISA: 1:16,000 Immunohistochemistry (paraffin): 3.75ug/ml. Heat induced antigen retrieval in citrate buffer, pH 6.0 is required. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to 15aa, TSGSVFPQQTGQLAE-C, from near the N-terminus of HAMP.
Form
Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human HAMP.
