149985
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
E WBCrossreactivity
Hu Mo RtShipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-DHX58 (Probable ATP-dependent RNA Helicase DHX58, Probable ATP-dependent Helicase LGP2, Protein D11Lgp2 Homolog, RIG-I-like Receptor 3, RLR-3, RIG-I-like Receptor LGP2, RLR, D11LGP2E, LGP2)
Anti-viral innate immune responses are triggered by pathogen-associated molecular patters (such as the accumulation of intracellular nucleic acids resulting from virus infections) and represent the first line of defense against numerous infectious organisms. The Toll-like receptors (TLR) 3, 7, 8, and 9 are expressed in immune cells and function as transmembrane pattern recognition receptors to detect foreign nucleic acids. Other proteins that play similar roles, such as RIG-1 and MDA5 are members of a CARD-helicase family and are expressed in the cytoplasm. A third protein, LGP2, is similar to RIG-1 and MDA5, except for lacking a homologous CARD domain. It is thought to act as an element of negative-feedback regulation of intracellular antiviral signaling. At least four isoforms of LGP2 are known to exist.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1-2ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
Rat Kidney Tissue Lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to 11aa from human LGP2.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human LGP2. Species Crossreactivity: mouse and rat..
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Akira S, Uematsu S, and Takeuchi O. Pathogen recognition and innate immunity. Cell2006; 124:783-801. 2. Iwasaki A and Medzhitov R. Toll-like receptor control of the adaptive immune responses. Nat. Immunol.2004; 5:987-95. 3. Yoneyama M, Kikuchi K, Matsumoto K, et al. The RNA helicase RIG-I has an essential function in double-stranded RNA-induced innate antiviral responses. Nat. Immunol.2004; 5:730-7. 4. Andrejeva J, Childs KS, Young DF, et al. The V proteins of paramyxoviruses bind the IFN-inducible RNA helicase, mda-5, and inhibits its activation of the IFN-betta promoter. Proc. Natl. Acad. Sci. USA, 2004; 101:17264-9.USBio References
No references available