Technical Data

151524
Clone Type
Polyclonal
Host
Goat
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Mo Rt
Accession #
NP_001477.1
Shipping Temp
Blue Ice
Storage Temp
-20°C
Goat Anti-GCKR (Glucokinase Regulatory Protein, Glucokinase Regulator, FGQTL5)

GCKR belongs to the SIS (Sugar ISomerase) family of proteins. The gene product is a regulatory protein that inhibits glucokinase in liver and pancreatic islet cells by binding non-covalently to form an inactive complex with the enzyme. The GCKR gene is considered a susceptibility gene candidate for a form of maturity-onset diabetes of the young (MODY).

Applications
Suitable for use in Peptide ELISA and Western Blot. Other applications not tested.
Recommended Dilutions
Peptide ELISA: 1:8000 Western Blot: 0.1-0.3ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
Rat Liver Tissue Lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to 16aa, KRFQHVIETPEPGK -C, from near the N-terminus of GCKR. Species Sequence Homology: human
Form
Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Purity
Purified by immunoaffinity chromatography
Specificity
Recognizes GCKR at ~70kD. Species Crossreactivity: mouse and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Kathiresan S, Melander O, Guiducci C, Surti A, Burtt NP, Rieder MJ, Cooper GM, Roos C, Voight BF, Havulinna AS, Wahlstrand B, Hedner T, Corella D, Tai ES, Ordovas JM, Berglund G, Vartiainen E, Jousilahti P, Hedblad B, Taskinen MR, Newton-Cheh C, Salomaa V, Peltonen L, Groop L, Altshuler DM, Orho-Melander M. Six new loci associated with blood low-density lipoprotein cholesterol, high-density lipoprotein cholesterol or triglycerides in humans. Nat Genet. 2008 Feb;40(2):189-97. Epub 2008 Jan 13.
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net