Goat Anti-CDKN2B (CDK inhibitory Protein, CDK4B inhibitor, CDK4I, cyclin-dependent kinase 4 inhibitor B, cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4), cyclin-dependent kinases 4 and 6 binding Protein, INK4B, MTS2, MTS-2, multiple tumor suppressor 2, p14_CDK inhibitor, p14_INK4B, p14-INK4b, P15, p15 CDK inhibitor, p15_INK4B, p15-INK4b, TP15, p15INK4b)
This gene lies adjacent to the tumor suppressor gene CDKN2A in a region that is frequently mutated and deleted in a wide variety of tumors. This gene encodes a cyclin-dependent kinase inhibitor, which forms a complex with CDK4 or CDK6, and prevents the activation of the CDK kinases, thus the encoded protein functions as a cell growth regulator that controls cell cycle G1 progression. The expression of this gene was found to be dramatically induced by TGF beta, which suggested its role in the TGF beta induced growth inhibition. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:128,000 Western Blot: 1-3ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Peptide with sequence C-RQGARERGARP, from the C-terminal of the protein sequence according to (NP_004927.2, NP_511042.1).
Form
Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Purity
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human p15INK4b.
