Goat Anti-Ryk (ERK-3, Growth factor receptor, Kinase VIK, Met-related kinase, NYK-R, Receptor-like tyrosine kinase, Tyrosine-protein kinase RYK, Vik)
May be a coreceptor along with FZD8 of Wnt proteins, such as WNT1, WNT3, WNT3A and WNT5A. Involved in neuron differentiation, axon guidance, corpus callosum establishment and neurite outgrowth. In response to WNT3 stimulation, receptor C-terminal cleavage occurs in its transmembrane region and allows the C-terminal intracellular product to translocate from the cytoplasm to the nucleus where it plays a crucial role in neuronal development.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:32,000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Peptide with sequence C-HAKSKVEYKLGFQ, from the internal region of the protein sequence (NP_038677.3, NP_001036072.1). Species sequence homology: rat, canine, porcine, bovine.
Form
Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Purity
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human Ryk. Species Crossreactivity: mouse
