Goat Anti-USP6 (Deubiquitinating enzyme 6, HRP1, Hyperpolymorphic gene 1, Proto-oncogene TRE-2, TRE17, TRE2, Tre-2, Tre-2 oncogene, Ubiquitin carboxyl-terminal hydrolase 6, Ubiquitin specific peptidase 6, Ubiquitin specific peptidase 6 (Tre-2 oncogene), Ubiquitin specific protease 6 (Tre-2 oncogene), Ubiquitin thioesterase 6, Ubiquitin thiolesterase 6, Ubiquitin-specific protease, Ubiquitin-specific-processing protease 6, USP6, USP6-short)
Deubiquitinase with an ATP-independent isopeptidase activity, cleaving at the C-terminus of the ubiquitin moiety. Catalyzes its own deubiquitination. In vitro, isoform 2, but not isoform 3, shows deubiquitinating activity. Promotes plasma membrane localization of ARF6 and selectively regulates ARF6-dependent endocytic protein trafficking. Is able to initiate tumorigenesis by inducing the production of matrix metalloproteinases following NF-kappa-B activation.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:4000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Peptide with sequence C-HHIDLDVRTTLRNH, from the internal region (near N-terminal domain) of the protein sequence (NP_004496.2).
Form
Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Purity
Purified by ammonium sulfate precipitation.
Specificity
Recognizes USP6. Species Sequence Homology: human
