Mouse Anti-Ankyrin G (ANK-3, ankyrin 3, Ankryin G119, brain specific ankyrin G, CHANK3)
Ankyrins are a family of adaptor proteins that mediate the attachment of integral membrane proteins to the spectrin-actin based membrane skeleton (1). Ankyrins have binding sites for the beta subunit of spectrin and at least 12 families of integral membrane proteins. This linkage is required to maintain the integrity of the plasma membranes and to anchor specific ion channels, ion exchangers and ion transporters in the plasma membrane.
Applications
Western Blot. Other applications not tested.
Recommended Dilution
1ug/ml was sufficient for detection of Ankyrin-G in 20ug of rat brain membrane lysate and assayed by Western Blot using goat anti-mouse IgG:HRP as the secondary antibody. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Fusion protein 1000 C-terminal amino acids of human Ankyrin G encompassing all of Ankyrin G with the exception of Ankyrin repeats
Form
Supplied as a liquid in PBS pH7.4, 50% glycerol.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human Ankyrin G. Species Crossreactivity: Human, Mouse, Rat. Detects over 200kD.
