167578
Clone Type
MonoclonalHost
MouseSource
RatIsotype
IgG2bClone Number
S294A-6Grade
Affinity PurifiedApplications
IC WBCrossreactivity
Mo RtAccession #
NP_001028837.1Gene ID
25393Shipping Temp
Blue IceStorage Temp
-20°CMouse Anti-Brevican (BCAN, BEHAB, CSPG7, Brain enriched hyaluronan binding protein antibody)
Brevican is the most abundant chondroitin sulfate proteoglycan in the extracellular matrix of the adult brain. It is a member of the lectican family of aggregating extracellular matrix proteoglycans that bear chondroitin sulfate (CS) chains. It is highly expressed in the central nervous system and is thought to stabilize synapses and inhibit neural plasticity. Brevican is secreted from astrocytes and neurons as a 145kD core protein that bears up to three, covalently-linked, CS chains. It is also is secreted as a 145kD core protein without CS chains. When cleaved by extracellular glutamyl endopeptidases, the ADAMTSs, a 55kD N-terminal fragment is formed that contains the unique C terminal epitope EAMESE
Applications
Western Blot and Immunocytochemistry. Other applications not tested.
Recommended Dilution
1ug/ml was sufficient for detection of Brevican in 20ug of rat brain lysate by Western Blot using Goat anti-mouse IgG:HRP as the secondary antibody. Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Fusion protein amino acids 219-655 of rat Brevican,|Accession# P55068.
Form
Supplied as a liquid in PBS pH7.4, 50% glycerol.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes rat Brevican. Species Crossreactivity: Rat, Mouse. Detects ~140kD (and smaller due to proteolytic cleavage)
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Yamada H., Watanabe K., Shimonaka M. and Yamaguchi Y. (1994) J. Biol. Chem. 269: 10119-10126.|2. Yamada H., Watanabe K., Shimonaka M., Yamasaki M.|and Yamaguchi Y. (1995) Biochem. Biophys. Res.|Commun. 216: 957-963.|3. Rauch U., et al. (1997) Genomics 44: 15-21.|4. Zhang H., Kelly G., Zerillo C., Jaworski D.M. and|Hockfield S. (1998) J. Neurosci. 7: 2370-2376.|5. Aspberg A., Adam S., Kostka G., Timpl R. and|Heinegard D. (1999) J. Biol. Chem. 274: 20444-20449.USBio References
No references available