Mouse Anti-GIT1 (ARF GAP GIT1, ARF GTPase-activating protein antibody, CAT1, GRK-interacting protein 1)
G-protein coupled receptor (GPCR) kinase interacting proteins 1 and 2 (GIT-1 and GIT-2) are highly conserved, ubiquitous scaffold proteins involved in localized signaling to help regulate focal contact assembly and cytoskeletal dynamics. GIT proteins contain multiple interaction domains that allow interaction with small GTPases (including ARF, Rac and cdc42), kinases (such as PAK and MEK), the Rho family GEF PIX, and the focal adhesion protein paxillin (reviewed in 1). GIT-1 is localized to focal adhesions, cytoplasmic complexes and membrane protrusions, and regulates cell protrusion formation and cell migration (2). GIT-1 has also been implicated in neuronal functions including synapse formation (3) and the pathology of Huntington disease 4). Huntington disease is a genetic neurodegenerative condition involving a mutation in the huntington gene.
The huntington gene product (htt) is ubiquitinated and degraded in human Huntington disease brains (5). Htt interacts directly with GIT-1 causing enhanced htt proteolysis, indicating that GIT-1 distribution and function may contribute to Huntington disease pathology (4).
Applications
Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry. Other applications not tested.
Recommended Dilution
1ug/ml was sufficient for detection of GIT1 in 10ug of rat brain lysate by Western Blot using Goat anti-mouse IgG:HRP as the secondary antibody Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Fusion protein amino acids
Form
Supplied as a liquid in PBS pH7.4, 50% glycerol.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes GIT1. Species Crossreactivity: Human, Mouse, Rat ~90kD. Does not cross-react
