167594
Clone Type
MonoclonalHost
MouseSource
RatIsotype
IgG2aClone Number
S1-12Grade
Affinity PurifiedApplications
IC IF IHC IP WBCrossreactivity
Hu Mo RtAccession #
NP_599190Gene ID
171373Shipping Temp
Blue IceStorage Temp
-20°CMouse Anti-KCC2 (Potassium chloride transporter 5, SLC12A5)
KCC2 is a member of the cation-chloride cotransporter gene family. It acts as a K-Cl cotransporter. KCCs normally lower intracellular chloride concentrations below the electrochemical equilibrium potential and depending on the chemical concentration gradients of potassium and chloride, KCC2 can operate as a net efflux or influx pathway. It is proposed to act as the main chloride extruder to promote fast hyperpolarizing postsynaptic inhibition in the brain. KCC2 is expressed at high levels in neurons throughout the nervous system and immunofluorescence shows that the protein is localized at inhibitory synapses of the spinal cord. Studies in mice have shown that KCC2 reduces GABA's inhibitory signaling, resulting in motor defects, epilepsy, and anxiety-like behavior.
Applications
Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry/Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000. 1ug/ml was sufficient for detection of KCC2 in 10ug of rat brain lysate using IgG (HRP) goat anti-mouse as the secondary antibody. Immunohistochemistry: 1:300 Immunofluorescence (IC): 1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Fusion protein corresponding to aa932-1043 from rat KCC2 Cellular Localization: membrane
Form
Supplied as a liquid in PBS pH 7.4, 0.09% sodium azide, 50% glycerol.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes rat KCC2 at ~140kD. Species Crossreactivity: human and mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Lee L.H., Walker J.A., Williams J.R., Goodier R.J., Payne J.A., Moss S.J. (2007) J Biol Chem. 282(41): 29777-29784. 2. Watanabe M., Wake H., Moorhouse A.J., Nabekura J. (2009) J Biol Chem. 284(41): 27980-27988. 3. Gulyas A.I., Sik A., Payne J.A., Kaila K., Freund T.F. (2001) Eur J Neurosci. 13(12): 2205-2217. 4. Vinay L., Jean-Xavier C. (2008) Brain Res Rev. 57(1): 103-110USBio References
No references available