Mouse Anti-SUR2A (Sulfonylurea receptor 2 antibody, CMD10, ABC37.)
Sulfonylurea receptors (SUR) are membrane proteins which are the molecular targets of the sulfonylurea class of anti-diabetic drugs whose mechanism of action is to promote insulin release from pancreatic beta cells. More specifically, SUR proteins are subunits of the inward-rectifier potassium ion channels Kir6.x (6.1 and 6.2) (1). The association of four K ir6.x and four SUR subunits form an ion conducting channel commonly referred to as the K ATP channel. The primary function of the sulfonylurea receptor is to sense intracellular levels of the nucleotides ATP and ADP and in response facilitate the open or closing its associated Kir 6.x potassium channel. Hence the K ATP channel monitors the energy balance within the cell (2).
Applications
Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended Dilution
1ug/ml was sufficient for detection of SUR2A in 20ug of mouse brain membrane lysate and assayed by Western Blot using goat anti-mouse IgG:HRP as the secondary antibody. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Fusion protein amino acids 1505-1546|(SSIVDAGLVLVFSEGILVECDTGPNLLQH|KNGLFSTLVMTNK, cytoplasmic C-terminus) of|mouse SUR2A, Accession# P70170.
Form
Supplied as a liquid in PBS pH7.4, 50% glycerol.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes mouse SUR2A. Species Crossreactivity: Rat, Mouse. Detects ~120kD. Does not cross-react with SUR2B
