Mouse Anti-Synaptotagmin-7 (P65, SVP65, DKFZp781D2042, SYT)
Synaptotagmins constitute a family of membrane trafficking proteins that are characterized by an N-terminal transmembrane region (TMR), a variable linker, and two C-terminal C2 domains - C2A and C2B. There are 15 members in the mammalian synaptotagmin family. There are several C2-domain containing protein families that are related to synaptotagmins, including transmembrane (Ferlins, E-Syts, and MCTPs) and soluble (RIMs, Munc13s, synaptotagmin-related proteins and B/K) proteins.
The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin participates in triggering neurotransmitter release at the synapse. The first domain mediates Ca(2+)-dependent phospholipid Synaptotagmins constitute a family of membrane trafficking proteins that are characterized by an N-terminal transmembrane region (TMR), a variable linker, and two C-terminal C2 domains - C2A and C2B. There are 15 members in the mammalian synaptotagmin family. There are several C2-domain containing protein families that are related to synaptotagmins, including transmembrane (Ferlins, E-Syts, and MCTPs) and soluble (RIMs, Munc13s, synaptotagmin-related proteins and B/K) proteins.
The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin participates in triggering neurotransmitter release at the synapse. The first domain mediates Ca(2+)-dependent phospholipid
Applications
Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended Dilution
1ug/ml was sufficient for detection of Synaptotagmin-7 in 20ug of rat brain lysate by Western Blot using Goat anti-mouse IgG:HRP as the secondary antibody. Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Fusion protein amino acids 150-239 (Cytoplasmic C2A domain) of mouse Synaptotagmin-7, Accession# Q9R0N7.
Form
Supplied as a liquid in PBS pH7.4, 50% glycerol.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes mouse Synaptotagmin-7. Species Crossreactivity: Rat, Mouse. Detects ~45kD. Does not cross-react with Synaptotagmin-6 (or others). Can identify other isoforms bands at ~65kD.
