167622

Sheep Anti-AMBP (a1-Microglobulin, A1M, EDC1, HCP, HI30, IATIL, ITI, ITIL, ITILC, UTI)

Human a1­Microglobulin (alpha1­m/A1M; also protein HC) is a secreted, 31­32kD glycoprotein member of the lipocalin family, calycin superfamily of molecules. It is expressed by hepatocytes, keratinocytes, and endodermal derivatives in the embryo. A1M appears to act as a heme scavenger, protecting cells and collagen against oxidative damage. It also acts as an immunosuppressant, inhibiting polyclonal lymphocyte activation and dampening granulocyte migration in response to chemokines. A1M circulates either as a monomer, or bound to IgA, albumin or prothrombin. Human A1M is generated through cleavage of a precursor molecule termed AMBP. This AMBP should not be confused with AMBP­1, a 120-140kD adrenomedullin­binding protein that is also known as Complement Factor H. The AMBP precursor contains a 19aa signal sequence, an N­terminal 183aa A1M protein (aa20-203), and a C­terminal serine protease inhibitor termed bikunin (aa206­352). A1M possesses one lipocalin domain (aa42-186). Although cleavage of AMBP in the Golgi apparatus typically generates a 31kD A1M and 28kD bikunin molecule, the 60­65kD AMBP precursor can also be released intact. A1M will undergo extracellular processing, generating a 30kD isoform that is missing aa199-203. There is one splice variant that shows a deletion of aa48­57. Over aa20­203, human A1M shares 76%aa sequence identity with both mouse and rat A1M.