Goat Anti-AP2A (A-adaptin A, Adaptor protein complex AP-2 a-1 subunit, Clathrin assembly protein complex 2a-A large chain, 100kD coated vesicle protein A, plasma membrane adaptor HA2/AP2)
AP-2 complex subunit alpha-1, Adapter-related protein complex 2 alpha-1 subunit, Adaptor protein complex AP-2 subunit alpha-1 Alpha1-adaptin, Plasma membrane adaptor HA2/AP2 Clathrin assembly protein complex 2 alpha-A large chain
The AP2A1 and AP2A2 represent the AP2-a proteins that are found in the AP2 complex in clathrin-coated vesicles. AP2A is also known as A-adaptin A, Adaptor protein complex AP-2 a-1 subunit, Clathrin assembly protein complex 2a-A large chain, 100kD coated vesicle protein A and plasma membrane adaptor HA2/AP2 adaptin a A subunit. The AP2 complex is a heterotetramer consisting of two large adaptins (a or b), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles, which link clathrin to receptors in vesicles.
Applications
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution
ELISA: 1:5000-1:20,000 Western Blot: 1:500-1:2000 Immunoprecipitation: 1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa3-14 from human AP2A1 and AP2A2. Species Sequence Homology: chimpanzee, orangutan, mouse, rat, chicken and canine.
Form
Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human AP2A.
