Rabbit Anti-ATG7 (APG7L, Ubiquitin-like Modifier-activating Enzyme ATG7, ATG12-activating Enzyme E1 ATG7, Autophagy-related Protein 7, Ubiquitin-activating Enzyme E1-like Protein)
APG7 autophagy 7-like (S. cerevisiae) antibody, APG7 like antibody, APG7, S. cerevisiae, homolog of antibody, APG7L antibody, ATG 7 antibody, atg7 antibody, ATG7 autophagy related 7 homolog (S. cerevisiae) antibody
Apg7 is the human gene product similar in homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae Apg7. In the yeast, the protein appears to be required for fusion of peroxisomal and vaculuolar membranes. The protein shows homlogy to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes.
Applications
Suitable for use in Immunofluorescence, ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:15,000-1:75,000 Western Blot: 1:500-1:2000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa550-575 from human Apg7.
Form
Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Apg7.