Mouse Anti-BUB1 (Mitotic Checkpoint Serine/Threonine-protein Kinase BUB1, hBub1, BUB1A, BUB1L)
Budding uninhibited by benzimidazoles 1 homolog antibody, hBUB1 antibody, Mitotic checkpoint serine/threonine protein kinase BUB1 antibody
BUB1(also called Mitotic checkpoint serine/threonine-protein kinase BUB1and BUB1A) is a kinase involved in spindle checkpoint function. The kinase functions in part by phosphorylating BUB3, a member of the miotic checkpoint complex, and activating the spindle checkpoint. Mutations in this gene have been associated with aneuploidy and several forms of cancer. BUB1 is autophosphorylated when cells enter mitosis. This protein is localized to the nucleus in interphase cells. Kinetochore localization is required for normal mitotic timing and checkpoint response to spindle damage. BUB1 is highly expressed in testis and thymus, less in colon, spleen, lung and small intestine. Expression is associated with cells/tissues with a high mitotic index.
Applications
Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution
Immunofluorescence: 1:500-1:2000 ELISA: 1:5000-1:20,000 Western Blot: 1:500-1:2000 Immunoprecipitation: 1:200 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to aa281-419 from human BUB1.
Form
Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human BUB1.
