Rabbit Anti-c-erbB2 (Receptor tyrosine-protein kinase erbB-2 p185erbB2 C-erbB-2 NEU proto-oncogene tyrosine kinase-type cell surface receptor HER2 MLN 19 CD340)
The ERBB2 gene encodes a member of the tyrosine protein kinase superfamily that is an essential component of a neuregulin-receptor complex called c-erbB2 (also known as Receptor tyrosine-protein kinase erbB-2, p185erbB2, C-erbB-2, NEU proto-oncogene, tyrosine kinase-type cell surface receptor HER2, MLN 19 and CD340), although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. C-erbB2 is not activated by EGF, TGF-a and amphiregulin. Defects in ERBB2 are associated with gastric cancer, familial glioma of brain (glioblastoma multiforme), ovarian cancer and lung cancer (adenocarcinoma of lung). c-erbB2 is ideal for Cancer and Signal Transduction research.
Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
ELISA: 1:10,000 Western Blot: 1:200-1:500 Immunohistochemistry (formalin fixed/paraffin embedded): 1:100, deparaffinize slides using xylene or xylene alternatives and graded alcohols. Staining requires boiling of sections in 10mM citrate buffer pH 6.0 for 10min followed by cooling at RT for 20 min. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to amino acid residues from the extracellular domain of human c-erbB2.
Form
Supplied as a liquid in TBS, 0.1% BSA, 0.09% sodium azide.
Specificity
Recognizes human c-erbB2.
