Technical Data

168140
Clone Type
Polyclonal
Host
Rabbit
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-DBP (DBP A, Decorin Binding Protein A )
Decorin-binding Protein A, Borrelia burgdorferi dbpA

This product is made against DbpA, or Decorin Binding Protein A from the spirochete Borrelia burgdorferi, which is carried by Ixodes ticks. DbpA from other microbial organisms such as E. coli (ATP-dependent RNA helicase DbpA) are significantly different. The spirochete migrates from the tick midgut during tick feeding to tick salivary glands and are thus transmitted to the mammal host. This transition may be facilitated by changes in expression of some B. burgdorferi genes. Spirochetal surface adhesions mediate attachment to decorin, a major component of the host extracellular matrix, enabling bacteria to colonize in mammalian tissues. It is believed that expression of the various proteins associated with the spirochete may be regulated by the changes in tick life cycle, changes in conditions during tick feeding (such as temperature, pH, and nutrients) and/or in coordination with the course of infection of the mammal host.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:5000 Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
MBP-fusion protein corresponding to Borrelia burgdorferi Dbp-A.
Form
Supplied as a lyophilized powder in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide. Reconstitute with sterile dH2O or PBS.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes Borrelia burgdorferi Dbp-A.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
No references available
USBio References
No references available
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