Rabbit Anti-NKIP1 (iASSP, inhibitor of apoptosis stimulating protein of p53, Inhibitor of ASPP protein, NFkB interacting protein 1, PPP1R13B-like protein)
inhibitor of apoptosis stimulating protein of p53 antibody, Inhibitor of ASPP protein antibody, NFkB interacting protein 1 antibody, NKIP1 antibody, PPP1R13B-like protein antibody
ASPP proteins (ASPP1, ASPP2 and iASPP) represent a new family of p53 binding proteins. ASPP1 and ASPP2 bind and enhance p53-mediated apoptosis. In contrast, the third member, iASPP, functionally inactivates p53. iASPP (also called protein phosphatase 1 regulatory (inhibitor) subunit 13 like protein, Inhibitor of ASPP protein, Protein iASPP, PPP1R13B-like protein and NFkB-interacting protein 1) plays a central role in regulation of apoptosis and transcription via its interaction with NF-k-B and p53/TP53 proteins. iASPP blocks transcription of HIV-1 virus by inhibiting the action of both NF-k-B and SP1.
Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:40,000-1:160,000 Western Blot: 1:1000-1:5000 Immunohistochemistry: 1:1000-1:5000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa780-797 from human iASPP (isoform 1).
Form
Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human iASSP.
