Rabbit Anti-PARK-7 (PARK7, Parkinson disease (autosomal recessive, early onset) 7, DJ-1 mutant, and DJ-1 oncogene product)
PARK7, Parkinson disease (autosomal recessive, early onset) 7, DJ-1 mutant, and DJ-1 oncogene product
The product of the PARK7 gene, known as PARK7, Parkinson disease (autosomal recessive, early onset) 7, DJ-1 mutant, and DJ-1 oncogene product, functions as a positive regulator of androgen receptor-dependent transcription. PARK7 may also function as a redox-sensitive chaperone and as a sensor for oxidative stress and also has been reported to prevent aggregation of SNCA. PARK7 protects neurons against oxidative stress and cell death and plays a role in fertilization. While PARK7 has no proteolytic activity, it does have a weak transforming activity. PARK7 forms a homodimer and binds to DJBP and PIAS2. PARK7 is part of a ternary complex containing PARK7, DJBP and AR and shows both a nuclear and cytoplasmic localization. In some cells, PARK7 is associated with mitochondria.
Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:10,000-1:50,000 Western Blot: 1:500-1:2000 Immunohistochemistry: 2mg/ml-5ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa177-189 from human PARK7.
Form
Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human PARK7. Species Crossreactivity: chimpanzee, african green monkey and zebrafish.
