Rabbit Anti-PUMA (BCL2 Binding Component 3, JFY 1, JFY1, p53 Up Regulated Modulator of Apoptosis)
Apoptosis is related to development as well as to many diseases. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members. This family of proteins also includes Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
Applications
Suitable for use in Immunocytochemistry, ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1-2ug/ml, a band at ~23kD can be detected. A lower band at ~16kD was detected in MOLT4 and U937 cell lysates, which may represent the PUMA-b form Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to amino acids near the C-terminus of human PUMA-a.
Form
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Purity
Purified by immunoaffinity chromatography from whole serum.
Specificity
Recognizes human PUMA. Species Crossreactivity: mouse.
