Rabbit Anti-RFFL (RING Finger and FYVE-like Domain-containing Protein 1, E3 Ubiquitin-protein Ligase Rififylin, Caspase Regulator CARP2, Caspases-8 and -10-associated RING Finger Protein 2, CARP2, CARP-2, FYVE-RING Finger Protein Sakura, Fring, RING Finger Protein 189, RNF189, RING Finger Protein 34-like, RNF34L)
RFFL, Rififylin, RING finger and FYVE-like domain-containing protein 1, FYVE-RING finger protein, Sakura, Fring, Caspases-8 and -10-associated RING finger protein 2, CARP-2, Caspase regulator CARP2, RING finger protein 189 and RING finger protein 34-like
RFFL, also known as rififylin, RING finger and FYVE-like domain-containing protein 1, FYVE-RING finger protein, sakura, fring, caspases-8 and -10-associated RING finger protein 2, CARP-2, caspase regulator CARP2, RING finger protein 189 and RING finger protein 34-like, is a novel modulator of NF-kB activation. RFFL possesses E3 ubiquitin protein ligase activity and has been shown to regulate the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. RFFL also possesses apoptotic activity and may bind phosphatidylinositol phosphates. RFFL is a membrane bound cytoplasmic protein that is expressed ubiquitously. RFFL can be detected in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes and is rapidly degraded after stimulation with TNFSF10, and probably by caspases. Multiple transcript variants have been detected for this protein.
Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:4,000-1:20,000 Western Blot: 1:500-1:3000 Immunohistochemistry: 1:500-1:3000 Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to aa1-363 from human RFFL.
Form
Supplied as a lyophilized powder in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide. Reconstitute with sterile dH2O or PBS.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human RFFL.
