Rabbit Anti-Tlk1 (PKU beta, serine threonine protein kinase, Serine/threonine protein kinase tousled like 1, TLK1, Tousled like kinase 1)
PKU beta antibody, serine threonine protein kinase antibody, Serine/threonine protein kinase tousled like 1 antibody, TLK1 antibody, Tousled like kinase 1 antibody
Tlk-1 (also known as tousled-like kinase, serine/threonine-protein kinase tousled-like 1 and PKU-beta) is a target for Chk1 upon induction of DNA-damage. Tousled-like kinases, first described in arabadopsis, are nuclear serine/threonine kinases that are potentially involved in the regulation of chromatin assembly. Tlk-1 is rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent. Tlk-1 heterodimerizes with Tlk-2. Multiple isoforms of the protein have been detected. Mature Tlk-1 is reported to have an apparent molecular weight of~86kD,~89kD and~64kD corresponding to isoforms 1, 2 and 3 respectively.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:10,000-1:50,000 Western Blot: 1:200-1:2000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa737-751 from human Tlk1.
Form
Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Purity
Purified by Protein A/G affinity chromatography.
Specificity
Recognizes human TIk1. Species Crossreactivity: chimpanzee, orangutan and canine.
