Rabbit Anti-TP53BP2 (Tumor Suppressor p53-binding Protein 2, 53BP2, p53-binding Protein 2, p53BP2, Apoptosis-stimulating of p53 Protein 2, ASPP2, Bcl2-binding Protein, Bbp, Renal Carcinoma Antigen NY-REN-51, PPP1R13A)
Apoptosis stimulating of p53 protein 2 antibody, Bbp antibody, Bcl2 binding protein antibody, NY REN 51 antigen antibody, p53 binding protein 2 antibody
ASPP (ankyrin-repeat-, SH3-domain-proline-rich-region protein)proteins (ASPP1, ASPP2 and iASPP)represent a new family of p53 binding proteins. ASPP1 and ASPP2 bind and enhance p53-mediated apoptosis. In contrast, iASPP functionally inactivates p53. ASPPs may also regulate p63- and p73-mediated apoptosis. Both ASPP1 and 2 directly interact with p53 and specifically enhance the apoptotic function of p53 by stimulating its DNA binding and transactivation function on promoters of pro-apoptotic genes, such as Bax and PIG-3. Not all cell cycle arrest genes are affected, such as p21. Interestingly, expression of ASPP is frequently down-regulated in human breast carcinomas expressing wild-type p53 but not mutant p53. Therefore, ASPP might regulate the tumor suppression function of p53 in vivo.
Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:2500-1:10,000 Western Blot: 1:500-1:3000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to human ASPP2.
Form
Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human ASPP2.
