Rabbit Anti-Wnt10B (Wnt10b, Wingless-type MMTV integration site family 10b, Wnt12)
Wnt10b, Wingless-type MMTV integration site family 10b, Wnt12
Wnt10b is a member of the Wnt family, a gene family that encodes secreted signaling proteins that play crucial roles in normal development such as regulation of cell fate and patterning during embryogenesis as well as neoplastic transformation. Wnt10b is found in the mouse mammary tumor virus insertion site where it is activated and causes mammary tumors. Elevated levels of Wnt10b have also been detected in human breast carcinomas. Wnt10b is known to be involved in adipogenesis, maintaining the preadipocyte in an undifferentiated state. More recently, Wnt10b has been shown to promote epithelial cell differentiation and hair shaft growth, demonstrating that Wnt10b has multiple roles in cell growth and differentiation.
Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:10,000-1:40,000 Western Blot: 2-4ug/ml Immunohistochemistry: 2.5ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to internal region of human Wnt10b.
Form
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Wnt10B. Two isoforms of WNT10b are known to exist; this antibody will only recognize the longer isoforms. This WNT10b antibody will not cross-react with WNT10a. Species Crossreactivity: mouse and rat.
