168670-AF-PE
Clone Type
MonoclonalHost
MouseSource
HumanConjugate
PEIsotype
IgG1,kClone Number
C1A/711Grade
PurifiedApplications
FC FLISA IF IHC IP WBCrossreactivity
HuGene ID
909 (Human)Shipping Temp
Blue IceStorage Temp
4°C Do Not FreezeNotes
Preservative Free
BSA Free
Mouse Anti-CD1a (Cortical thymocyte antigen CD1A, Epidermal dendritic cell marker CD1a antibody, FCB6, HTA1, T cell surface antigen T6 / Leu 6, T-Cell Surface Glycoprotein CD1A) (PE)
(Cortical thymocyte antigen CD1A, Epidermal dendritic cell marker CD1a antibody, FCB6, HTA1, T cell surface antigen T6 / Leu 6, T-Cell Surface Glycoprotein CD1A)
At least five CD1 genes (CD1a, b, c, d, and e) are identified. CD1 proteins have been demonstrated to restrict T cell response to non-peptide lipid and glycolipid antigens and play a role in non-classical antigen presentation. CD1a is a non-polymorphic MHC Class 1 related cell surface glycoprotein, expressed in association with Beta-2 microglobulin. Anti-CD1a labels Langerhans cell histiocytosis (Histiocytosis X), extranodal histiocytic sarcoma, a subset of T-lymphoblastic lymphoma/leukemia, and interdigitating dendritic cell sarcoma of the lymph node. When combined with antibodies against TTF-1 and CD5, anti-CD1a is useful in distinguishing between pulmonary and thymic neoplasms since CD1a is consistently expressed in thymic lymphocytes in both typical and atypical thymomas, but only focally in 1/6 of thymic carcinomas and not in lymphocytes in pulmonary neoplasms. Anti-CD1a is reported to be a new marker for perivascular epithelial cell tumor (PEComa).
Applications: Suitable for use inImmunofluorescence, Flow Cytometry, FLISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.
Recommended Dilution
FLISA: For coating, order Ab without BSA Flow Cytometry: 0.5-1ug/million cells Immunofluorescence: 1-2ug/ml Immunoprecipitation: 1-2ug/500ug protein Western Blot: 0.5-1ug/ml Immunohistochemistry: Frozen & formalin-fixed: 0.5-1ug/ml for 30 min at RT (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes Optimal dilutions to be determined by the researcher.
Positive Control
MOLT-4 cells. Paracortex in a tonsil or a reactive lymph node.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Recombinant human CD1a protein. Cellular Localization: Cell surface
Form
Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with R-Phycoerythrin (PE).
Purity
Purified by Protein A/G affinity chromatography.
Specificity
Recognizes human CD1a.
