Technical Data

168800-AF-ML650
Clone Type
Monoclonal
Host
Mouse
Conjugate
MaxLight™650
Isotype
IgG1,k
Clone Number
HIV1-24/661
Grade
Affinity Purified
Applications
FLISA IC IF
Gene ID
155348 (HIV-1)
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free
Mouse Anti-Human Immunodeficiency Virus Type-1 p24 (Capsid protein p24, Human immunodeficiency virus type 1 p24, HIV1gp1, HIV-1 Gag p24) (BSA & Azide Free) (MaxLight 650)
(Capsid protein p24, Human immunodeficiency virus type 1 p24, HIV1gp1, HIV-1 Gag p24)

MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.

Human immunodeficiency virus (HIV) is a retrovirus that causes acquired immune deficiency syndrome (AIDS), a condition in humans in which the immune system begins to fail, leading to life-threatening opportunistic infections. HIV mainly infects vital cells in the human immune system such as helper T cells (specifically CD4+ T cells), macrophages and dendritic cells. Two species of HIV infect humans: HIV-1 and HIV-2, with HIV-1 being the more virulent strain. The gag gene of human immunodeficiency virus 1 (HIV-1) encodes a precursor protein known as Pr55Gag. The viral protease PR cleaves this precursor to generate p17, p24, p7, and p6 proteins, which are required for virus particle assembly. HIV-1 Gag p24 is a capsid protein that constitutes the core of AIDS virus HIV-1. p6 and p7 are the components of the nucleocapsid, and p17 provides a protective matrix. HIV-1 Gag p24 is indispensable to the reproduction of AIDS virus and constitutes an essential element for the AIDS virus particle construction. As this protein is detectable from the early stage of AIDS virus infection, its measurement is commonly used as an indicator of HIV-1 infection and viral load.
Applications:  Suitable for use in Immunofluorescence, FLISA and Immunocytochemistry. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Positive Control
HIV-1 infected cells or tissues.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Recombinant HIV-1 Gag p24 protein. Swissprot: P04585.|Cellular Localization: Membrane
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
Purity
Purified by Protein A/G affinity chromatography.
Specificity
Recognizes Human Immunodeficiency Virus Type-1 p24.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Voltersvik, P., Bostad, L., Dyrhol-Riise, A.M., Eide, G.E., Rosok, B.I., Olofsson, J. and Asjö, B. 2006. Cystatin A and HIV-1 p24 antigen expression in tonsil- lar lymphoid follicles during HIV-1 infection and during highly active anti- retroviral therapy. J. Acquir. Immune Defic. Syndr. 41: 277-2784.
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net