Mouse Anti-Kappa Light Chain (HCAK1, Ig Kappa Chain C Region, IGKC, Immunoglobulin KM)
(HCAK1, Ig Kappa Chain C Region, IGKC, Immunoglobulin KM)
This MAb is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
Applications
Suitable for use in ELISA, Flow Cytometry, Immunohistochemistry. Other applications not tested.
Recommended Dilution
Flow Cytometry: 0.5-1ug/million cells Immunohistochemistry: Frozen: 0.5-1.0ug/ml for 30 min at RT Optimal dilutions to be determined by the researcher.Optimal dilutions to be determined by the researcher.
Positive Control
293T, Raji or hPBL cells. Tonsil or Spleen
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Human IgG-kappa myeloma protein. Cellular Localization: Cell Surface, cytoplasmic and secreted
Form
Supplied as a liquid in 10mM PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 168814-AF.
Purity
Purified by Protein A/G affinity chromatography.
Specificity
Recognizes human Kappa Light Chain.