Mouse Anti-TGF-alpha (EGF-like TGF, ETGF, TFGA, TGF Type 1, TGFA, Wa1, Waved 1) (BSA & Azide Free)
(EGF-like TGF, ETGF, TFGA, TGF Type 1, TGFA, Wa1, Waved 1)
The staining with this MAb is completely blocked by the peptide used for raising this antibody. TGF-alpha (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.
Applications: Suitable for use in Immunofluorescence, ELISA, Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Flow Cytometry: 0.5-1ug/million cells Immunofluorescence: 1-2ug/ml Immunohistochemistry (Formalin-paraffin): 2-4ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes Optimal dilutions to be determined by the researcher.
Positive Control
Jurkat or Ramos cells, Testicular carcinoma
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa34-43, PPVAAAVVSH of human TGF-alpha. Cellular Localization: Cytoplasmic and extracellular (secreted)
Form
Supplied as a liquid in 10mM PBS. Also available with BSA and azide, see 168869.
Purity
Purified by Protein A/G affinity chromatography.
Specificity
Recognizes human TGF-alpha. Species Crossreactivity: rabbit and zebrafish. Does not react with EGF and the neuropeptide synenkephalin.
