VEGF-R1 (Fms like tyrosine kinase 1 (Flt-1), Fms-like tyrosine kinase 1, FRT, Tyrosine protein kinase FRT, Tyrosine protein kinase receptor FLT, Vascular endothelial growth factor receptor 1 (VEGFR1), Vascular permeability factor receptor 1) (BSA & Azide Free) (MaxLight 550)

168886-AF-ML550

Clone Type

Monoclonal

Host

Mouse

Source

Human

Conjugate

MaxLight™550

Isotype

IgG1,k

Clone Number

FLT1/658

Grade

Affinity Purified

Applications

FLISA

Crossreactivity

Gene ID

2321 (Human)

Shipping Temp

Blue Ice

Storage Temp

4°C Do Not Freeze

Notes

Preservative Free
BSA Free

Mouse Anti-VEGF-R1 (Fms like tyrosine kinase 1 (Flt-1), Fms-like tyrosine kinase 1, FRT, Tyrosine protein kinase FRT, Tyrosine protein kinase receptor FLT, Vascular endothelial growth factor receptor 1 (VEGFR1), Vascular permeability factor receptor 1) (BSA & Azide Free) (MaxLight 550)

(Fms like tyrosine kinase 1 (Flt-1), Fms-like tyrosine kinase 1, FRT, Tyrosine protein kinase FRT, Tyrosine protein kinase receptor FLT, Vascular endothelial growth factor receptor 1 (VEGFR1), Vascular permeability factor receptor 1)

MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.

Three cell membrane receptor tyrosine kinases, Flt-1 (also designated VEGF-R1), Flk-1 (also designated VEGF-R2) and Flt-4, putatively involved in the growth of endothelial cells, are characterized by the presence of seven immunoglobulin-like sequences in their extracellular domain. These receptors exhibit high degrees of sequence relatedness to each other as well as lesser degrees of relatedness to the class III receptors including CSF-1/Fms, PDGR, SLFR/Kit and Flt-3/Flk-2. Two members of this receptor class, Flt-1 and Flk-1, have been shown to represent high affinity receptors for vascular endothelial growth factors (VEGFs). On the basis of structural similarity to Flt-1 and Flk-1, it has been speculated that Flt-4 might represent a third receptor for either VEGF or a VEGF-related ligand.

Applications: Suitable for use in FLISA, Functional Studies. Other applications not tested.

Recommended Dilution

Optimal dilutions to be determined by the researcher.

Positive Control

293FT or A431 cells. Cerebellum or skin cancer.

Storage and Stability

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Immunogen

Recombinant human VEGF-R1 protein. Cellular Localization: Cell surface

Form

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.

Purity

Purified by Protein A/G affinity chromatography.

Specificity

Recognizes human VEGF-R1 (FLT-1).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References

1. Shibuya, M., Yamaguchi, S., Yamane, A., Ikeda, T., Tojo, A., Matsushime, H. and Sato, M. 1990. Nucleotide sequence and expression of a novel human receptor-type tyrosine kinase gene (Flt) closely related to the Fms family. Oncogene 5: 519-524. 2. De Vries, C., Escobedo, J.A., Ueno, H., Houck, K., Ferrara, N. and Williams, L.T. 1992. The Fms-like tyrosine kinase, a receptor for vascular endothelial growth factor. Science 255: 989-991.

USBio References

No references available

Technical Data