Technical Data

169179
Clone Type
Monoclonal
Host
Rat
Source
Mouse
Isotype
IgG2b
Clone Number
33A10
Grade
Purified
Applications
FC IF IHC WB
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rat Anti-Plet-1 (Placenta expressed transcript-1)

The 33A10-defined antigen is the orphan protein, Placenta expressed transcript (Plet)-1. Biochemical data indicated that Plet-1 is a glycosylphosphatidylinositol-anchored glycoprotein with N-linked carbohydrates in addition to other posttranslational modifications. Plet-1 is a a recently identified protein that as of yet has no known function and is not associated with any one family of proteins. Plet-1 is used as a cell surface marker present on stem/progenitor epithelial cells and in a number of tissues including thymus and skin, and on epithelial cell types in mammary gland, prostate, and pancreas.

Applications
Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Mouse mammary tumor cells
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified
Specificity
Recognizes mouse Plet-1. Reactivity is identified in the developing mouse mammary gland, in the bronchioles of the lung, the salivary glands and the skin. Plet-1 is a product specific for keratinocytes undergoing terminal trichilemmal differentiation in the hair follicle and is expressed at the plasma membranes.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. A Sonnenberg, H Daams, M A Van der Valk, J Hilkens and J Hilgers (1986), J Histochem Cytochem 34(8): 1037-1046. 2. Karine Raymond, Anja Richter, Maaike Kreft, Evelyne Frijns, Hans Janssen, Monique Slijper, Silke Praetzel-Wunder, Lutz Langbein and Arnoud Sonnenberg (2010), J Invest Derm 130 1500-1513.
USBio References
No references available
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