Technical Data

171512
Clone Type
Monoclonal
Host
Rat
Source
Human
Isotype
IgM
Clone Number
38.13
Grade
Purified
Applications
FC IC IHC
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rat Anti-CD77

CD77 (globotriaosylceramide Gb3), also known as the Pk blood group antigen, BLA (Burkitt´s lymphoma associated antigen), or CTH (ceramide trihexoside) is a neutral glycosphingolipid composed of three carbohydrate molecules linked to a lipid moiety in the cell membrane (Gal-alpha1-4Gal-beta1-4Glc-beta1-Cer). It is expressed on germinal center B cells, Burkitt´s lymphoma cells, it can be induced on extrafolicular B cells and it is also found on endothelia and epithelia. CD77 may be involved in elimination of germinal center B cells that fail to produce high affinity antibodies, and serves also as receptor for shiga toxin and verotoxin.

Applications
Suitable for use in Immunocytochemistry, Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Flow Cytometry: 1ug/ml Immunohistochemistry: frozen Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Daudi cell line (Burkitt´s lymphoma)
Form
Supplied as a liquid in TBS, pH 8.0, 0.09% sodium azide.
Purity
Purified
Specificity
Recognizes human CD77.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Wiels, J., et al., (1981), “Monoclonal antibody against a Burkitt lymphoma-associated antigen”, Proc.|Nat. Acad. Sci. USA, 78, 6485-6488. 2. Wiels, J., et al., (1984), “Properties of immunotoxins against a glycolipid antigen associated with Burkitt's lymphoma”, Cancer Res., 44, 129-133. 3. Nudelman, E., et al., (1983), “A glycolipid antigen associated with Burkitt lymphoma defined by a monoclonal antibody”, Science, 220, 509-511. 4. Fellous, M., et al., (1985), “A monoclonal antibody against a Burkitt lymphoma associated antigen has an anti-Pk red blood cell specificity”, Brit.J.Haematol. 60, 559-565. 5. Wiels, J., Tursz, T., (1989), “BLA a glycolipid marker of Burkitt's lymphoma and a subset of germinal-centre B-cells”, in Leucocyte Typing IV, Knapp, W., Ed. Oxford University Press.
USBio References
No references available
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