Mouse Anti-CD31 (EndoCAM, PECA1, Platelet Endothelial Cell Adhesion Molecule 1, GPIIA', PECAM-1) (BSA & Azide Free)
EndoCAM, PECA1, Platelet Endothelial Cell Adhesion Molecule 1, GPIIA', PECAM-1
CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.
Applications
Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.
Recommended Dilution
Flow Cytometry: 0.5-1ug/million cells Immunofluorescence: 0.5-1ug/ml Western Blot: 0.5-1.0ug/ml Immunoprecipitation: 0.5-1µg/500ug protein lysate Immunohistochemistry (Frozen & Formalin-fixed): 0.5-1.0ug/ml for 30 minutes at RT (Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined.
Positive Control
Tonsil, Angiosarcoma
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Membrane preparation of a spleen from a patient with hairy cell leukemia (JC/70A). Cellular Localization: Cell surface and cytoplasm of endothelial cells
Form
Supplied as a liquid in PBS. Also available with BSA and azide, see 172045.
Purity
Purified by Protein A/G affinity chromatography.
Specificity
Recognizes CD31. Species Crossreactivity: Human, Cynomolgus Monkey and Rabbit. Does not react with rat and procine.