Technical Data

172075-AF-ML650
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
MaxLight™650
Isotype
IgG1
Clone Number
SPM265
Grade
Affinity Purified
Applications
FC FLISA IF IHC IP WB
Crossreactivity
Hu
Gene ID
3875 (Human)
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free
Mouse Anti-Cytokeratin 18 (Cell Proliferation-inducing Gene 46 Protein, CK18, CYK18 Cytokeratin Endo B, K18, Keratin-18, Kerd, KRT18) (BSA & Azide Free) (MaxLight 650)
Cell Proliferation-inducing Gene 46 Protein, CK18, CYK18 Cytokeratin Endo B, K18, Keratin-18, Kerd, KRT18

MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.

This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.
Applications
Suitable for use in Immunofluorescence, Flow Cytometry, FLISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.
Recommended Dilution
Flow Cytometry: 0.5-1ug/million cells Immunofluorescence: 1-2ug/ml Immunoprecipitation: 1-2ug/500ug protein Western Blot: 0.5-1ug/ml Immunohistochemistry (Frozen & Formalin-fixed): 0.5-1ug/ml for 30 min at RT (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined.
Positive Control
MCF-7, HeLa, A431 cells or Breast Cancer
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. 
Note: Applications are based on unconjugated antibody.
Immunogen
PMC-42 human breast carcinoma cells. Cellular Localization: Cytoplasmic
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
Purity
Purified by Protein A/G affinity chromatography.
Specificity
Recognizes Cytokeratin 18. Species Crossreactivity: Human. Does not react with mouse, rat, sheep, hamster, bovine, dog & pig.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Kovarik J et al. Int J Cancer, Supplement. 3:50-55 (1988). 2. Lane EB, Alexander CM. Sem Cancer Biol 1:165-179 (1990).
USBio References
No references available
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