172103-AF-ML550
Clone Type
MonoclonalHost
MouseSource
HumanConjugate
MaxLight™550Isotype
IgG1Clone Number
SPM250Grade
Affinity PurifiedApplications
FC FLISA IF IHCCrossreactivity
HuShipping Temp
Blue IceStorage Temp
4°C Do Not FreezeNotes
Preservative Free
BSA Free
Mouse Anti-Granulocyte Marker (Pluripoietin, Filgrastim, Lenograstim, granulocyte colony-stimulating factor, G-CSF) (BSA & Azide Free) (MaxLight 550)
Pluripoietin, Filgrastim, Lenograstim, granulocyte colony-stimulating factor, G-CSF
MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.
Recognizes an unidentified antigen in the cytoplasm of mature granulocytes. It shows no reactivity with any other cell type in human tissues. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. MAb BM-2 reacts with early precursor and mature forms of human myeloid cells. This MAb is useful for the detection of myeloid leukemias and granulocytic sarcomas. It can be used as a marker of granulocytes in normal tissues or inflammatory processes.
Applications
Suitable for use in Immunofluorescence, Flow Cytometry, FLISA, Immunohistochemistry. Other applications not tested.
Recommended Dilution
Optimal dilution for a specific application should be determined.
Positive Control
Tonsil or lymph node
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Nuclei from pokeweed mitogen stimulated human peripheral blood lymphocytes. Cellular Localization: Cytoplasmic
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
Purity
Purified by Protein A/G affinity chromatography.
Specificity
Recognizes human Granulocyte Marker. Species Crossreactivity: macaque monkey.
