172132-AF-FITC
Clone Type
MonoclonalHost
MouseSource
HumanConjugate
FITCIsotype
IgG1,kClone Number
SPM563Grade
Affinity PurifiedApplications
FC FLISA IHC WBCrossreactivity
Ch Hu Mo Po RtGene ID
4744 (Human); 24587 (Rat)Shipping Temp
Blue IceStorage Temp
-20°CNotes
Preservative Free
BSA Free
Mouse Anti-Neurofilament (NEFH, Neurofilament H, Neurofilament Heavy Polypeptide 200kD, Neurofilament Triplet H Protein, NF-H, NF200, NF-H) (FITC)
NEFH, Neurofilament H, Neurofilament Heavy Polypeptide 200kD, Neurofilament Triplet H Protein, NF-H, NF200, NF-H
This MAb reacts with a 200kD protein, identified as heavy sub-unit of neurofilaments (NF-H). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kD (NF-L), 160kD (NF-M) and 200kD (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.
Applications
Suitable for use in Flow Cytometry, FLISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Immunohistochemistry (Frozen & Formalin-fixed): Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 minutes at 95ºC followed by cooling at RT for 20 minutes. Optimal dilutions to be determined by the researcher.
Positive Control
Brain, Neuroblastoma
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Human recombinant neurofilament protein. Cellular Localization: Cytoplasm, cytoskeleton, cell projection and axon
Form
Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified by Protein A/G affinity chromatography.
Specificity
Recognizes human Neurofilament. Species Crossreactivity: mouse, rat, porcine and chicken.
