Rabbit Anti-PARP1 (ARTD1, Poly(ADP-ribose) Polymerase 1, ADP-ribosyltransferase Diphtheria Toxin-like 1, NAD(+) ADP-ribosyltransferase 1)
PARP-1 (ARTD1) is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. PARP-1 positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. It forms a complex with EEF1A1 and TXK that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production.
Applications
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1:2000-1:5000 Immunohistochemistry: 1:500-1:1000 Immunocytochemistry: 1:500-1:1000 Immunoprecipitation: 2ul/100ug nuclear extract Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to aa215-525 from human PARP-1.
Form
Supplied as a liquid in 0.09% sodium azide.
Specificity
Recognizes human PARP-1. Cross-reacts weakly with mouse and monkey PARP-1
