Goat Anti-ARNT (aa69-82) (Aryl hydrocarbon receptor nuclear translocator, BHLHe2, Class E basic helix-loop-helix protein 2, Dioxin receptor, Nuclear translocator, HIF1B, HIF1BETA, HIF1-beta, HIF-1beta, HIF-1-beta, Hypoxia-inducible factor 1, beta subunit, OTTHUMP00000218217, OTTHUMP00000218218, OTTHUMP00000218220, TANGO)
Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia.
Applications
Suitable for use in Peptide ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Peptide ELISA: 1:4000 Western Blot: 2-4ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa69-82 from sequence CNDKERFARSDDEQS, at the internal region (near N-terminus) of the protein sequence according to NP_001659.1. Species sequence homology: mouse, rat, canine and bovine
Form
Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human ARNT. This antibody is expected to recognize all reported isoform 1 (NP_001659.1) only.
