Calcium-sensitive protein which reversibly blocks the barbed ends of actin filaments but does not sever preformed actin filaments. May play an important role in macrophage function. May play a role in regulating cytoplasmic and/or nuclear structures through potential interactions with actin. May bind DNA.
Applications
Suitable for use in Peptide ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Peptide ELISA: 1:64,000 Western Blot: 0.3-1ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-NIRATERALNWDS, from the internal region of the protein sequence according to NP_001738.2; NP_001243069.1. Species sequence homology: canine
Form
Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human CAPG. This antibody is expected to recognize both reported isoforms (NP_001738.2; NP_001243069.1). Reported variants represent identical protein: NP_001243068.1, NP_001738.2.