Goat Anti-CTDSP1 (SCP1, NLIIF, CTD (Carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase 1, Small CTD phosphatase 1, Nuclear LIM interactor-interacting factor, Synaptonemal Complex Protein 1)
CTDSP1 is a class 2C phosphatase with activity dependent on the conserved DxD motif. Expression of CTDSP1 inhibited activated transcription from several promoter-reporter gene constructs, but expression of a mutant lacking phosphatase activity enhanced transcription. Neuronal gene transcription is repressed in nonneuronal cells by the repressor element-1 (RE1)-silencing transcription factor/neuron-restrictive silencer factor (REST/NRSF; 600571) complex. REST/NRSF recruits SCPs to neuronal genes that contain RE1 elements, leading to neuronal gene silencing in nonneuronal cells. Phosphatase-inactive forms of SCP interfere with REST/NRSF function and promote neuronal differentiation of P19 stem cells. Likewise, small interfering RNA directed to the single Drosophila SCP unmasks neuronal gene expression in S2 cells. Thus, SCP activity is an evolutionarily conserved transcriptional regulator that acts globally to silence neuronal genes.
Applications
Suitable for use in Peptide ELISA, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilution
Peptide ELISA: 1:16,000 Western Blot: 0.1-0.3ug/ml Immunohistochemistry: 5-10ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to EAKAQDSDKIC, from the internal region of the protein sequence according to NP_067021.1; NP_872580.1. Species sequence homology: rat, canine and bovine
Form
Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes CTDSP1. This antibody is expected to recognize both reported isoforms (NP_067021 and NP_872580). Species Crossreactivity: human and mouse
