Rabbit Anti-CYP11A1 (Cytochrome P450 11A1, CYP11A, CYPXIA1, P450SCC)
The formation of biologically active steroid hormones is significantly influenced by the motrochondrial cytochrome P450 enzyme, chrolesterol side-chain cleavage. CYP11A1 functions in catalyzing the first step of steroid biosynthesis under modulation of cAMP.It ia also known as cytochrome P450C11A1, cytochrome P450scc and cytochrome P450, subfamily XIA. Expression of CYP11A1 has been a useful tool for monitoring the differentiation state of cells from varius endocrine tissues. Identification of CYP11A1 expression by 13363-1-AP detected a ~45kD band in human placenta, with respect to the reported band at 49-52kD by Roby, et al and Durkee, et al.
Applications
Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 1:500-1:2000 Immunohistochemistry: 1:50-1:200 Immunofluorescence: 1:50-1:200 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to human CYP11A1.
Form
Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Purity
Purified by affinity chromatography.
Specificity
Recognizes human CYP11A1. Species Crossreactivity: rat
