Rabbit Anti-SETD2 (SET Domain-containing Protein 2, Histone-lysine N-methyltransferase SETD2, HYPB, SET2, HIF-1, HIP-1, KMT3A, HBP231, HSPC069, p231HBP)
Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons, is caused by an expansion of a polyglutamine tract in the HD protein huntingtin. This gene encodes a protein belonging to a class of huntingtin interacting proteins characterized by WW motifs. This protein is a histone methyltransferase that is specific for lysine-36 of histone H3, and methylation of this residue is associated with active chromatin. This protein also contains a novel transcriptional activation domain and has been found associated with hyperphosphorylated RNA polymerase II.
Applications
Suitable for use in Immunofluorescence/Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, ELISA and Western Blot. Other applications not tested.
Recommended Dilutions
Western Blot: 1:500-1:1000 Immunohistochemistry (Paraffin) : 1:500-1:1000. High pressure antigen retrieval performed with citrate buffer, pH 6.0 is recommended. Immunofluorescence (IC): 1:50-1:200 Immunoprecipitation: 0.5ug-4ug for 400ug-600ug extracts of whole cells Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to aa2475-2564 of human SETD2.
Form
Supplied as a liquid in PBS, pH 7.3, 0.05% Proclin-300, 50% glycerol.
Purity
Purified by affinity chromatography.
Specificity
Recognizes human SETD2. Species Crossreactivity: mouse and rat
