Technical Data

214464
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
4C2
Grade
Affinity Purified
Applications
IF WB
Crossreactivity
Hu Mo Po Rb Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-Actin, beta (ACTB, Beta Actin)

Actins are highly conserved proteins that are involved in cell motility, structure and integrity and are ubiquitously expressed in all eukaryotic cells. Six different isoforms of actin have been identified in mammals. (Vandekerckhove et al. 1978).

Mouse anti Human actin beta, has been used to look at the remodeling of the actin cytoskeleton following sendai virus infection and the specific role of the viral M protein in actin remodelling (Miazza et al. 2011)
Applications
Suitable for use in Immunohistochemistry, Western Blot and Immunofluorescence. Other applications not tested.
Recommended Dilution
Immunohistochemistry (Frozen): 1:50-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to the acetylated N-terminal nonapeptide of beta-cytoplasmic actin, coupled to KLH.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography from tissue culture supernatant.
Specificity
Recognizes human Actin Beta. Species Crossreactivity: Mouse, porcine, rabbit and rat. Highly specific for cytoplasmic actin beta and does not react with other actin isoforms.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Dugina, V. et al. (2009) Beta and gamma­cytoplasmic actins display distinct distribution and functional diversity. J. Cell Sci. 155: 2980­2988 2. Brockmann, C. et al. (2011) Beta­ and gamma­cytoplasmic actins are required for meiosis in mouse oocytes. Biol Reprod. 85: 1025­39. 3. Miazza, V. et al. (2011) Sendai virus induced cytoplasmic actin remodeling correlates with efficient virus particle production. Virology. 410: 7­16. 4. Baranwal, S. et al. (2012) Nonredundant roles of cytoplasmic b­ and y­actin isoforms in regulation of epithelial apical junctions. Mol Biol Cell. 23: 3542­53. 5. Shagieva, G.S. et al. (2012) Actin Isoforms and Reorganization of Adhesion Junctions in Epithelial­Mesenchymal Transition of Cervical Carcinoma Cells Biochemistry (Moscow) BM12­154 6. Perrin, B.J. and Ervasti, J.M. (2010) The Actin Gene Family: Function Follows Isoform Cytoskeleton (Hoboken). 67: 630­4.
USBio References
No references available
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