Mouse Anti-Amikacin (MaxLight 650)
MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.
Recognizes Amikacin, an aminoglycoside antibiotic. Amikacin binds to the 30S ribosomal subunit of microbial RNA, disrupting mRNA reading and protein synthesis. It may be used to treat severe infection with multiple drug resistant Gram negative bacteria such as Enterobacter. Potential side effects include kidney damage and hearing loss.
Applications
Suitable for use in FLISA. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
Purity
Purified by Protein A affinity chromatography from ascites.
Specificity
Recognizes Amikacin.
